293T/B2M KO Cell Line

Description: B2M KO HEK293T cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, verified by flow cytometry (FACS) and Sanger sequencing.

Gene Name: B2M

Gene Full Name: beta-2-microglobulin

Accession Number: NM_004048.4

Target Sequence: Sequence available upon order placement

Quantity: Two vials of frozen cells (5×106 per vial)

Stability: Stable in culture over a minimum of 10 passages

Application: Drug screening and biological assays

Freezing Medium: 70% DMEM+20% FBS+10% DMSO

Propagation Medium: DMEM+10%FBS

Morphology: Epithelial

Subculture: Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 105 cells/mL

Incubation: 37 °C with 5% CO2

Storage: Liquid nitrogen immediately upon receiving

Doubling Time: Approximately 30 hours

Mycoplasma Status: Negative

Caution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.