CAR-T/CAR-NK Development

Expanding Immunotherapy Frontiers: Allogeneic CAR-T and CAR-NK Cells for Safer, Universal Cancer Treatment

Immunotherapy is transforming cancer treatment, with CAR-T cell therapy emerging as a groundbreaking approach for hematologic malignancies such as leukemia and lymphoma. To expand its therapeutic reach, researchers are developing allogeneic (off-the-shelf) CAR-T cells by disrupting genes like TCR to prevent graft-versus-host disease (GvHD).

In parallel, CAR-engineered natural killer (CAR-NK) cells are gaining momentum as an alternative platform. Unlike T cells, NK cells do not require prior activation and can recognize tumor cells independent of MHC expression—making them ideal for universal, allogeneic applications. CAR-NK therapies also tend to have a more favorable safety profile, with reduced risk of cytokine release syndrome (CRS) and neurotoxicity.

With deep expertise in tumor immunology, RedyBio provides comprehensive CRO services to accelerate your CAR-T, CAR-NK, and other immune cell therapy programs. Our services include:

Monoclonal antibody development
CAR construct design and vector production
CAR lentivirus/Retrovirus manufacturing
Immune cell isolation, activation, and transduction
Phenotypic and functional analysis
In vitro cytotoxicity assays
In vivo efficacy models

RedyBio Custom Immune Cell Therapy Research Services

With deep expertise in tumor immunology, RedyBio provides comprehensive CRO services to accelerate your CAR-T, CAR-NK, and other immune cell therapy programs. Our services include:

Antibody Development

Animal immunization
Hybridoma generation and screening
Subcloning and large-scale amplification
Hybridoma sequencing
Functional assay validation

CAR Design & Lentivirus Production

Custom CAR molecule design and vector construction
High-titer CAR lentiviral/retroviral vector production
Stable cell line generation for target antigen expression

Immune Cell Preparation

Human T and CAR-T cell preparation
NK and CAR-NK cell generation
Allogeneic (“universal”) CAR-T cell production
Murine CAR-T cell services

Phenotypic Characterization

T cell analysis: activation, exhaustion, memory subsets
NK cell surface marker profiling
Myeloid and other immune cell phenotyping

In Vitro Efficacy Assays

CAR-T/NK cell proliferation assays
Target cell cytotoxicity analysis
Chronic antigen stimulation assay
ADCC (antibody-dependent cellular cytotoxicity) assays
ADCP (antibody-dependent cellular phagocytosis) assays
Multiplex cytokine detection

In Vivo Efficacy Studies

CDX (cell line-derived xenograft) tumor models using immunodeficient mice
Syngeneic mouse tumor models
Humanized immune system models (PBMC and HSC-based)
Dosing studies and tumor growth monitoring
Biochemical and histological analysis of tissues and samples

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Reach out for more information or a quote. Our team is here to assist you with your research needs and inquiries.