K562/Firefly Luciferase Cell Line

Description: The K562 cell line was derived from a patient with chronic myelogenous leukemia (CML) in terminal blast crisis. The K562 Cell Line does not express antigens CD19, CD20 or CD22, therefore it makes an excellent negative control in assays that measure specific killing by CAR-T cells targeting B cell antigens CD19, CD20, and CD22. It can also be used as a highly sensitive in vitro target for the natural killer assay.  The signal generated by the firefly luciferase reporter is proportional to cell numbers.

Quantity: Two vials of frozen cells (2×106 per vial)

Stability: Stable in culture over a minimum of 10 passages

Application: Drug screening, biological assays and in vivo study

Freezing Medium: 70% RPMI 1640 +20% FBS+10% DMSO

Propagation Medium: RPMI1640+10%FBS

Morphology: Lymphoblast

Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.

Incubation: 37 °C with 5% CO2

Storage: Liquid nitrogen immediately upon receiving

Doubling Time: Approximately 40 hours

Mycoplasma Status: Negative

Caution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.