NALM6/Firefly Luciferase Cell Line

Description: NALM6/Firefly Luciferase cells are derived from the human precursor B cell lymphoblast cell line NALM6. NALM6 cells stably express CD19, are ideal for testing CD19 CAR T cells or other immunotherapies.  NALM6/firefly luciferase cells stably express firefly luciferase, which can be used for in vitro assays and in vivo studies.

Quantity: Two vials of frozen cells (2×106 per vial)

Stability: Stable in culture over a minimum of 10 passages

Application: Drug screening, biological assays and in vivo study

Freezing Medium: 50% RPMI 1640 +40% FBS+10% DMSO

Propagation Medium: RPMI1640+10%FBS

Morphology: Lymphocyte-like

Subculture: Cultures can be maintained by addition or replacement of fresh medium. Start cultures between 2.0 X 105 cells/mL and 4.0 X 105 cells/mL maintain between 1.0 X 105 and 1.0 X 106 cells/mL.

Incubation: 37 °C with 5% CO2

Storage: Liquid nitrogen immediately upon receiving

Doubling Time: Approximately 36 hours

Mycoplasma Status: Negative

Caution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.