Off-target DNA & Library Prep, Sequencing and Data Analysis (per sample)

This service package starts from cell samples, including genomic DNA extraction, library preparation, NGS, data analysis and final report in PowerPoint format. For this service, the customers usually has designed their gRNA sets, transfected target cells, and collect cells for next step analysis. The following is a list of steps included in this service package.

• gDNA extraction, sonication and QC2

• Library preparation and QC3

• NGS sequencing and data collecting

• NGS data trimming and QC

• Sequencing data alignment

• Off-Target analysis

• Off-Target visualization and report generation