HCT 116/Firefly Luciferase Cell Line

Description: Human HCT 116 cell line was derived from human colorectal carcinoma. It has a mutation in codon 13 of the ras proto-oncogene. HCT 116 cells are positive for transforming growth factor beta 1 (TGF beta 1) and beta 2 (TGF beta 2) expression. HCT 116 cell line was used in tumorigenicity studies in vitro and in vivo. It forms primary tumor after implantation into nude mice, which makes it a great tool for studying the mechanisms of tumor growth and metastasis. HCT116/firefly luciferase stable cells express firefly luciferase.

Quantity: Two vials of frozen cells (2×106 per vial)

Stability: Stable in culture over a minimum of 10 passages

Application: Drug screening, biological assays and in vivo study

Freezing Medium: 70% DMEM +20% FBS+10% DMSO

Propagation Medium: DMEM+10%FBS

Morphology: Epithelial

Subculture: Split saturated culture 1:3-1:6 every 3 days

Incubation: 37 °C with 5% CO2

Storage: Liquid nitrogen immediately upon receiving

Doubling Time: Approximately 30 hours

Mycoplasma Status: Negative

Caution: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information.